A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

CBL-mediated targeting of CIPKs facilitates the decoding of calcium signals emanating from distinct cellular stores.

Batistic O, Waadt R, Steinhorst L, Held K, Kudla J

During adaptation and developmental processes cells respond through nonlinear calcium-decoding signaling cascades, the principal components of which have been identified. However, the molecular mechanisms generating specificity of cellular responses remain poorly understood. Calcineurin B-like (CBL) proteins contribute to decoding calcium signals by specifically interacting with a group of CBL-interacting protein kinases (CIPKs). Here, we report the subcellular localization of all ... [more]

Plant J. Jan. 01, 2010; 61(2);211-22 [Pubmed: 19832944]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CBL5 SOS2	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Hashimoto K (2012)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

CBL5

Gene Ontology Biological Process

Gene Ontology Molecular Function

calcium ion binding [ISS]

Gene Ontology Cellular Component

SOS2