BAIT

RPA2

ATRPA2, ATRPA32A, ROR1, RPA32A, SUPPRESSOR OF ROS1, T28I24.22, T28I24_22, replicon protein A2, AT2G24490
replicon protein A2
GO Process (3)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

RPA1A

ARABIDOPSIS THALIANA REPLICATION PROTEIN A 1A, ARABIDOPSIS THALIANA RPA70-KDA SUBUNIT A, ATRPA1A, ATRPA70A, RPA70-KDA SUBUNIT A, RPA70A, T12H3.6, T12H3_6, replication protein A 1A, AT2G06510
replication factor A 1A
GO Process (6)
GO Function (0)
GO Component (2)
Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Arabidopsis replication protein A 70a is required for DNA damage response and telomere length homeostasis.

Takashi Y, Kobayashi Y, Tanaka K, Tamura K

Replication protein A1 (RPA1/RPA70) forms a heterotrimeric complex together with RPA2/RPA32 and RPA3/RPA14 subunits which plays essential roles in various aspects of DNA metabolism including replication, repair, recombination and telomere maintenance. Compared with RPA70 in yeast and mammals, limited information is available about the factor in plants. In this study, we analyzed the functions of AtRPA70a, which is most similar ... [more]

Plant Cell Physiol. Nov. 01, 2009; 50(11);1965-76 [Pubmed: 19812063]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RPA2 RPA1A
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-
RPA2 RPA1A
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-

Curated By

  • BioGRID