BAIT

NRT1.1

ARABIDOPSIS THALIANA NITRATE TRANSPORTER 1, ATNRT1, ATNRT1.1, B-1, CHL1, CHL1-1, CHLORATE/NITRATE TRANSPORTER, CHLORINA 1, F12F1.1, F12F1_1, NITRATE TRANSPORTER 1, NITRATE/CHLORATE TRANSPORTER, NRT1, nitrate transporter 1.1, AT1G12110
nitrate transporter 1.1
GO Process (3)
GO Function (3)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

CIPK23

ATCIPK23, CBL-interacting protein kinase 23, F12P21.6, F12P21_6, LKS1, LOW-K+-SENSITIVE 1, SNF1-RELATED PROTEIN KINASE 3.23, SnRK3.23, AT1G30270
CBL-interacting serine/threonine-protein kinase 23
GO Process (4)
GO Function (3)
GO Component (5)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

CHL1 functions as a nitrate sensor in plants.

Ho CH, Lin SH, Hu HC, Tsay YF

Ions serve as essential nutrients in higher plants and can also act as signaling molecules. Little is known about how plants sense changes in soil nutrient concentrations. Previous studies showed that T101-phosphorylated CHL1 is a high-affinity nitrate transporter, whereas T101-dephosphorylated CHL1 is a low-affinity transporter. In this study, analysis of an uptake- and sensing-decoupled mutant showed that the nitrate transporter ... [more]

Cell Sep. 18, 2009; 138(6);1184-94 [Pubmed: 19766570]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CIPK23 NRT1.1
Biochemical Activity
Biochemical Activity

An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Low-BioGRID
343829
NRT1.1 CIPK23
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID