BAIT

SUT2

ARABIDOPSIS THALIANA SUCROSE TRANSPORTER 3, ATSUC3, ATSUT2, SUC3, SUCROSE TRANSPORTER 3, T17M13.3, T17M13_3, sucrose transporter 2, AT2G02860
sucrose transport protein SUC3
Arabidopsis thaliana (Columbia)
PREY

SUT4

ATSUC4, ATSUT4, F21M12.35, F21M12_35, SUC4, SUCROSE TRANSPORTER 4, AT1G09960
sucrose transporter 4
Arabidopsis thaliana (Columbia)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Interactions between co-expressed Arabidopsis sucrose transporters in the split-ubiquitin system.

Schulze WX, Reinders A, Ward J, Lalonde S, Frommer WB

BACKGROUND: The Arabidopsis genome contains nine sucrose transporter paralogs falling into three clades: SUT1-like, SUT2 and SUT4. The carriers differ in their kinetic properties. Many transport proteins are known to exist as oligomers. The yeast-based split ubiquitin system can be used to analyze the ability of membrane proteins to interact. RESULTS: Promoter-GUS fusions were used to analyze the cellular expression ... [more]

BMC Biochem. Mar. 18, 2003; 4(0);3 [Pubmed: 12689351]

Throughput

  • Low Throughput

Additional Notes

  • Split-ubiquitin assay

Curated By

  • BioGRID