BAIT

SUC2

ARABIDOPSIS THALIANA SUCROSE-PROTON SYMPORTER 2, ATSUC2, SUCROSE TRANSPORTER 1, SUCROSE-H+ SYMPORTER, SUT1, T22J18.12, T22J18_12, sucrose-proton symporter 2, AT1G22710
sucrose transport protein SUC2
GO Process (1)
GO Function (4)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

SUT2

ARABIDOPSIS THALIANA SUCROSE TRANSPORTER 3, ATSUC3, ATSUT2, SUC3, SUCROSE TRANSPORTER 3, T17M13.3, T17M13_3, sucrose transporter 2, AT2G02860
sucrose transport protein SUC3
Arabidopsis thaliana (Columbia)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Interactions between co-expressed Arabidopsis sucrose transporters in the split-ubiquitin system.

Schulze WX, Reinders A, Ward J, Lalonde S, Frommer WB

BACKGROUND: The Arabidopsis genome contains nine sucrose transporter paralogs falling into three clades: SUT1-like, SUT2 and SUT4. The carriers differ in their kinetic properties. Many transport proteins are known to exist as oligomers. The yeast-based split ubiquitin system can be used to analyze the ability of membrane proteins to interact. RESULTS: Promoter-GUS fusions were used to analyze the cellular expression ... [more]

BMC Biochem. Mar. 18, 2003; 4(0);3 [Pubmed: 12689351]

Throughput

  • Low Throughput

Additional Notes

  • Split-ubiquitin assay

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SUT2 SUC2
PCA
PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Low-BioGRID
430062

Curated By

  • BioGRID