BAIT

GYRA

DNA GYRASE A, T7M13.23, AT3G10690
DNA gyrase subunit A
Arabidopsis thaliana (Columbia)
PREY

GYRB1

DNA GYRASE B1, AT3G10270
DNA gyrase subunit B
Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Arabidopsis thaliana GYRB3 does not encode a DNA gyrase subunit.

Evans-Roberts KM, Breuer C, Wall MK, Sugimoto-Shirasu K, Maxwell A

BACKGROUND: DNA topoisomerases are enzymes that control the topology of DNA in all cells. DNA gyrase is unique among the topoisomerases in that it is the only enzyme that can actively supercoil DNA using the free energy of ATP hydrolysis. Until recently gyrase was thought to be unique to bacteria, but has now been discovered in plants. The genome of ... [more]

PLoS ONE Apr. 03, 2010; 5(3);e9899 [Pubmed: 20360860]

Throughput

  • Low Throughput

Curated By

  • BioGRID