BAIT

AT3G14990

protein DJ-1-like A
Arabidopsis thaliana (Columbia)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

The Arabidopsis DJ-1a protein confers stress protection through cytosolic SOD activation.

Xu XM, Lin H, Maple J, Bjoerkblom B, Alves G, Larsen JP, Moller SG

Mutations in the DJ-1 gene (also known as PARK7) cause inherited Parkinson's disease, which is characterized by neuronal death. Although DJ-1 is thought to be an antioxidant protein, the underlying mechanism by which loss of DJ-1 function contributes to cell death is unclear. Human DJ-1 and its Arabidopsis thaliana homologue, AtDJ-1a, are evolutionarily conserved proteins, indicating a universal function. To ... [more]

J. Cell. Sci. May. 15, 2010; 123(0);1644-51 [Pubmed: 20406884]

Throughput

  • Low Throughput

Curated By

  • BioGRID