BAIT

ROPGEF11

ATROPGEF11, F9I5.12, F9I5_12, PIRF1, RHO guanyl-nucleotide exchange factor 11, phytochrome interacting RopGEF 1, AT1G52240
RHO guanyl-nucleotide exchange factor 11
GO Process (2)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

A small GTPase Activator Protein Interacts with cytoplasmic phytochromes in regulating root development.

Shin DH, Cho MH, Kim TL, Yoo J, Kim JI, Han YJ, Song PS, Jeon JS, Bhoo SH, Hahn TR

Phytochromes enable plants to sense light information and regulate the developmental responses. Phytochromes interact with partner proteins to transmit light signals to downstream components for plant development. Phytochrome-interacting ROP (Rho-like GTPase of plant) guanine-nucleotide exchange factor (RopGEF11), PIRF1, functions as a light-signaling switch regulating root development through the activation of ROPs in the cytoplasm. In vitro pull-down and yeast two-hybrid ... [more]

Unknown Jun. 15, 2010; 0(0); [Pubmed: 20551316]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ROPGEF11 PHYA
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
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Curated By

  • BioGRID