A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

HHP1, a novel signalling component in the cross-talk between the cold and osmotic signalling pathways in Arabidopsis.

Chen CC, Liang CS, Kao AL, Yang CC

Heptahelical protein 1 (HHP1) is a negative regulator in abscisic acid (ABA) and osmotic signalling in Arabidopsis. The physiological role of HHP1 was further investigated in this study using transgenic and knock-out plants. In HHP1::GUS transgenic mutants, GUS activity was found to be mainly expressed in the roots, vasculature, stomata, hydathodes, adhesion zones, and connection sites between septa and seeds, ... [more]

J. Exp. Bot. Jul. 01, 2010; 61(12);3305-20 [Pubmed: 20566565]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
HHP1 ICE1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Chen CC (2010)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

HHP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

receptor activity [ISS]

Gene Ontology Cellular Component

ICE1