BAIT

AT4G14830

DL3455W, FCAALL.332
hypothetical protein
GO Process (0)
GO Function (1)
GO Component (1)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

CDPK1

ATCDPK1, CALCIUM-DEPENDENT PROTEIN KINASE, CPK10, F6A14.1, F6A14_1, calcium-dependent protein kinase 1, AT1G18890
calcium-dependent protein kinase 1
GO Process (3)
GO Function (4)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Arabidopsis Calcium-Dependent Protein Kinase AtCPK10 Functions in ABA and Ca2+-Mediated Stomatal Regulation in Response to Drought Stress.

Zou JJ, Wei FJ, Wang C, Wu JJ, Ratnasekera D, Liu WX, Wu WH

Plant calcium-dependent protein kinases (CDPKs) may function as calcium sensors and play important roles in regulation of plant growth and development and in plant responses to biotic and abiotic stresses. The Arabidopsis genome encodes 34 CDPKs and most of them have not been functionally characterized. Here we report the functional characterization of CPK10 in Arabidopsis response to drought stress. The ... [more]

Unknown Aug. 30, 2010; 0(0); [Pubmed: 20805328]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
AT4G14830 CDPK1
Reconstituted Complex
Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Low-BioGRID
-
CDPK1 AT4G14830
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID