An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

FERONIA receptor-like kinase regulates RHO GTPase signaling of root hair development.

Duan Q, Kita D, Li C, Cheung AY, Wu HM

Plant RHO GTPases (RAC/ROPs) mediate multiple extracellular signals ranging from hormone to stress and regulate diverse cellular processes important for polarized cell growth, differentiation, development, reproduction, and responses to the environment. They shuttle between the GDP-bound inactive state and the GTP-bound activated state and their activation is predominantly mediated by a family of guanine nucleotide exchange factors (GEFs) referred to ... [more]

Unknown Sep. 27, 2010; 0(0); [Pubmed: 20876100]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

ROP2

Gene Ontology Biological Process

Gene Ontology Molecular Function

GTP binding [ISS]

Gene Ontology Cellular Component

FER