Publication

In vitro nuclear interactome of the HIV-1 Tat protein.

Gautier VW, Gu L, O'Donoghue N, Pennington S, Sheehy N, Hall WW

BACKGROUND: One facet of the complexity underlying the biology of HIV-1 resides not only in its limited number of viral proteins, but in the extensive repertoire of cellular proteins they interact with and their higher-order assembly. HIV-1 encodes the regulatory protein Tat (86-101aa), which is essential for HIV-1 replication and primarily orchestrates HIV-1 provirus transcriptional regulation. Previous studies have demonstrated ... [more]

Throughput

• High Throughput

Additional Notes

• HIV 1: Protein TAT
• High Throughput: An in vitro GST-Tat pull-down assay was performed using nuclear extracts from Jurkat cells followed by mass spectrometric analysis to identify interacting proteins.

Curated By

• BioGRID