BAIT

AT1G25330

F4F7.28, F4F7_28
transcription factor CESTA
GO Process (2)
GO Function (2)
GO Component (1)
Arabidopsis thaliana (Columbia)
PREY

BEE1

BR enhanced expression 1, F15H18.11, F15H18_11, AT1G18400
transcription factor BEE 1
GO Process (3)
GO Function (3)
GO Component (1)
Arabidopsis thaliana (Columbia)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

CESTA, a positive regulator of brassinosteroid biosynthesis.

Poppenberger B, Rozhon W, Khan M, Husar S, Adam G, Luschnig C, Fujioka S, Sieberer T

Brassinosteroids (BRs) are steroid hormones that are essential for the development of plants. A tight control of BR homeostasis is vital for modulating their impact on growth responses. Although it is recognized that the rapid adaptation of de novo synthesis has a key role in adjusting required BR levels, our knowledge of the mechanisms governing feedback control is limited. In ... [more]

EMBO J. Mar. 16, 2011; 30(6);1149-61 [Pubmed: 21336258]

Throughput

  • Low Throughput

Curated By

  • BioGRID