BAIT

HIT1

ARABIDOPSIS THALIANA VPS53 HOMOLOG, ATVPS53, HEAT-INTOLERANT 1, VPS53, AT1G50500
VPS53 family protein HIT1
GO Process (4)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

POK

ARABIDOPSIS THALIANA VPS52 HOMOLOG, ATVPS52, F3I17.8, F3I17_8, POKY POLLEN TUBE, T-DNA TRANSMISSION DEFECT 8, TTD8, AT1G71270
Vps52 / Sac2-like protein
GO Process (2)
GO Function (0)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Involvement of the Arabidopsis HIT1/AtVPS53 tethering protein homologue in the acclimation of the plasma membrane to heat stress.

Wang LC, Tsai MC, Chang KY, Fan YS, Yeh CH, Wu SJ

Arabidopsis thaliana hit1-1 is a heat-intolerant mutant. The HIT1 gene encodes a protein that is homologous to yeast Vps53p, which is a subunit of the Golgi-associated retrograde protein (GARP) complex that is involved in retrograde membrane trafficking to the Golgi. To investigate the correlation between the cellular role of HIT1 and its protective function in heat tolerance in plants, it ... [more]

Unknown Mar. 11, 2011; 0(0); [Pubmed: 21398432]

Throughput

  • Low Throughput

Curated By

  • BioGRID