BAIT
PFT1
F2J7.4, F2J7_4, PHYTOCHROME AND FLOWERING TIME 1, AT1G25540
mediator of RNA polymerase II transcription subunit 25
GO Process (9)
GO Function (3)
GO Component (2)
Gene Ontology Biological Process
- defense response to fungus [IMP]
- jasmonic acid mediated signaling pathway [IMP]
- positive regulation of defense response [IMP]
- positive regulation of flower development [IMP]
- positive regulation of transcription, DNA-templated [IMP]
- red, far-red light phototransduction [IMP]
- regulation of flower development [IMP]
- response to far red light [IMP]
- response to red light [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- mediator complex [IDA]
- nucleus [IDA, ISM]
Arabidopsis thaliana (Columbia)
PREY
RRTF1
redox responsive transcription factor 1, AT4G34410
ethylene-responsive transcription factor ERF109
GO Process (5)
GO Function (2)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Arabidopsis thaliana (Columbia)
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
A High-Throughput Screening System for Arabidopsis Transcription Factors and Its Application to Med25-Dependent Transcriptional Regulation.
The activities of transcription factors (TFs) require interactions with specific DNA sequences and other regulatory proteins. To detect such interactions in Arabidopsis, we developed a high-throughput screening system with a Gateway-compatible Gal4-AD-TF library of 1589 Arabidopsis TFs, which can be easily screened by mating-based yeast-one-hybrid (Y1H) and yeast-two-hybrid (Y2H) methods. The efficiency of the system was validated by examining two ... [more]
Unknown Feb. 22, 2011; 0(0); [Pubmed: 21343311]
Throughput
- Low Throughput
Curated By
- BioGRID