Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Activity-dependent neuroprotective protein constitutes a novel element in the SWI/SNF chromatin remodeling complex.

Mandel S, Gozes I

Complete deficiency in activity-dependent neuroprotective protein (ADNP), a heterochromatin 1-binding protein, results in dramatic changes in gene expression, neural tube closure defects, and death at gestation day 9 in mice. To further understand the cellular roles played by ADNP, the HEK293 human embryonic kidney cell line that allows efficient transfection with recombinant DNA was used as a model for the ... [more]

J. Biol. Chem. Nov. 23, 2007; 282(47);34448-56 [Pubmed: 17878164]

Throughput

  • Low Throughput

Curated By

  • BioGRID