BAIT

AT5G53360

K19E1.16, K19E1_16
E3 ubiquitin-protein ligase SINAT5
GO Process (2)
GO Function (0)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

DET1

ATDET1, DE-ETIOLATED 1, FUS2, FUSCA 2, T9A4.17, AT4G10180
light-mediated development protein DET1
GO Process (2)
GO Function (0)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Ubiquitination of LHY by SINAT5 regulates flowering time and is inhibited by DET1.

Park BS, Eo HJ, Jang IC, Kang HG, Song JT, Seo HS

Ubiquitin is a small polypeptide and ubiquitination is the post-translational modification by ubiquitin protein, resulting in degradation of target proteins by the 26S proteasome complex. Here, we found that E3 ubiquitin ligase SINAT5, an Arabidopsis homologue of the Drosophila SINA RING-finger protein, interacts directly with LHY, a component of the circadian oscillator, and DET1, a negative regulator of light-regulated gene ... [more]

Biochem. Biophys. Res. Commun. Jul. 23, 2010; 398(2);242-6 [Pubmed: 20599732]

Throughput

  • Low Throughput

Curated By

  • BioGRID