BAIT

VPS2.3

F21M11.12, F21M11_12, VPS2, vacuolar protein sorting-associated protein 2.3, AT1G03950
vacuolar protein sorting-associated protein 2.3
GO Process (1)
GO Function (0)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

SNF7.1

F19B15.190, F19B15_190, VPS32, AT4G29160
vacuolar protein sorting-associated protein 32-2
GO Process (2)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

The Arabidopsis ESCRT protein-protein interaction network.

Shahriari M, Richter K, Keshavaiah C, Sabovljevic A, Huelskamp M, Schellmann S

In yeast, endosomal sorting of monoubiquitylated transmembrane proteins is performed by a subset of the 19 "class E vacuolar protein sorting" proteins. The core machinery consists of 11 proteins that are organised in three complexes termed ESCRT I-III (endosomal sorting complex required for transport I-III) and is conserved in eukaryotic cells. While the pathway is well understood in yeast and ... [more]

Unknown Mar. 26, 2011; 0(0); [Pubmed: 21442383]

Throughput

  • Low Throughput

Curated By

  • BioGRID