A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

AtMPK4 is required for male-specific meiotic cytokinesis in Arabidopsis.

Zeng Q, Chen JG, Ellis BE

Mitogen-activated protein kinase cascades have been implicated in regulating various aspects of plant development, including somatic cytokinesis. The evolution of expanded plant MAPK gene families has enabled the diversification of potential MAPK cascades, but functionally overlapping components are also well documented. Here we report that Arabidopsis MPK4, a mitogen-activated protein kinase that was previously described as a regulator of disease ... [more]

Unknown May. 16, 2011; 0(0); [Pubmed: 21575092]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
MKK6 MPK11	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Zeng Q (2011)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

MKK6

Gene Ontology Molecular Function

MAP kinase kinase activity [IDA, TAS]kinase activity [ISS]protein binding [IPI]

Gene Ontology Cellular Component

MPK11

Gene Ontology Biological Process