A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

CAST AWAY, a membrane-associated receptor-like kinase, inhibits organ abscission in Arabidopsis.

Burr CA, Leslie ME, Orlowski SK, Chen I, Wright CE, Daniels MJ, Liljegren SJ

Receptor-like kinase-mediated cell signaling pathways play fundamental roles in many aspects of plant growth and development. A pair of Arabidopsis thaliana leucine-rich repeat receptor-like kinases (LRR-RLKs), HAESA (HAE) and HAESA-LIKE2 (HSL2), have been shown to activate the cell separation process that leads to organ abscission. Another pair of LRR-RLKs, EVERSHED (EVR) and SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE1 (SERK1), act as inhibitors ... [more]

Unknown May. 31, 2011; 0(0); [Pubmed: 21628627]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

CONNEXIN 32

Gene Ontology Biological Process

Gene Ontology Molecular Function

kinase activity [ISS]protein binding [IPI]protein kinase activity [IDA]

Gene Ontology Cellular Component

HAE