SQN
Gene Ontology Biological Process
Gene Ontology Molecular Function
HSP81-2
Gene Ontology Biological Process
- chaperone-mediated protein complex assembly [IMP]
- defense response [IMP]
- defense response to bacterium, incompatible interaction [IMP]
- flower development [IMP]
- heat acclimation [IEP]
- leaf development [IMP]
- negative regulation of seed germination [IMP]
- protein folding [ISS]
- protein stabilization [IMP]
- response to heat [IEP]
- response to salt stress [IMP]
- response to water deprivation [IMP]
- stomatal closure [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Binding of SQUINT to Hsp90 is required for the function of this Cyclophilin 40-related protein in Arabidopsis.
SQUINT (SQN) is the Arabidopsis ortholog of the immunophilin, Cyclophilin 40 (Cyp40), and promotes miRNA activity by promoting the activity of AGO1. In animals and Saccharomyces cerevisiae, Cyp40 promotes protein activity in association with the protein chaperone Hsp90. To determine if Cyp40 also acts in association with Hsp90 in plants, we examined the interaction between SQN and Hsp90 in vitro, ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID