BAIT

AT2G33510

F4P9.28, F4P9_28
hypothetical protein
GO Process (0)
GO Function (0)
GO Component (1)

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

HDG1

HD-GL2-1, HOMEODOMAIN-GLABRA2 1, homeodomain GLABROUS 1, AT3G61150
homeodomain GLABROUS 1
GO Process (2)
GO Function (2)
GO Component (1)
Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

CFL1, a WW Domain Protein, Regulates Cuticle Development by Modulating the Function of HDG1, a Class IV Homeodomain Transcription Factor, in Rice and Arabidopsis.

Wu R, Li S, He S, Wassmann F, Yu C, Qin G, Schreiber L, Qu LJ, Gu H

Plants have a chemically heterogeneous lipophilic layer, the cuticle, which protects them from biotic and abiotic stresses. The mechanisms that regulate cuticle development are poorly understood. We identified a rice (Oryza sativa) dominant curly leaf mutant, curly flag leaf1 (cfl1), and cloned CFL1, which encodes a WW domain protein. We overexpressed both rice and Arabidopsis CFL1 in Arabidopsis thaliana; these ... [more]

Unknown Sep. 27, 2011; 0(0); [Pubmed: 21954461]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
HDG1 AT2G33510
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-

Curated By

  • BioGRID