An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Arabidopsis thaliana Nudix hydrolase AtNUDT7 forms complexes with the regulatory RACK1A protein and Ggamma subunits of the signal transducing heterotrimeric G protein.

Olejnik K, Bucholc M, Anielska-Mazur A, Lipko A, Kujawa M, Modzelan M, Augustyn A, Kraszewska E

Arabidopsis thaliana AtNUDT7 Nudix pyrophosphatase hydrolyzes NADH and ADP-ribose in vitro and is an important factor in the cellular response to diverse biotic and abiotic stresses. Several studies have shown that loss-of-function Atnudt7 mutant plants display many profound phenotypes. However the molecular mechanism of AtNUDT7 function remains elusive. To gain a better understanding of this hydrolase cellular role, proteins interacting ... [more]

Unknown Nov. 08, 2011; 0(0); [Pubmed: 22068106]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

NUDT7

Gene Ontology Biological Process

Gene Ontology Molecular Function

ADP-glucose pyrophosphohydrolase activity [IDA]ADP-ribose diphosphatase activity [IDA]ADP-ribose pyrophosphohydrolase activity [IDA]NAD binding [IDA]NAD+ diphosphatase activity [IDA]hydrolase activity [ISS]nucleoside-diphosphatase activity [IDA]protein binding [IPI]

Gene Ontology Cellular Component

TUB5