A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Arabidopsis thaliana Nudix hydrolase AtNUDT7 forms complexes with the regulatory RACK1A protein and Ggamma subunits of the signal transducing heterotrimeric G protein.

Olejnik K, Bucholc M, Anielska-Mazur A, Lipko A, Kujawa M, Modzelan M, Augustyn A, Kraszewska E

Arabidopsis thaliana AtNUDT7 Nudix pyrophosphatase hydrolyzes NADH and ADP-ribose in vitro and is an important factor in the cellular response to diverse biotic and abiotic stresses. Several studies have shown that loss-of-function Atnudt7 mutant plants display many profound phenotypes. However the molecular mechanism of AtNUDT7 function remains elusive. To gain a better understanding of this hydrolase cellular role, proteins interacting ... [more]

Unknown Nov. 08, 2011; 0(0); [Pubmed: 22068106]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

NUDT7

Gene Ontology Biological Process

Gene Ontology Molecular Function

ADP-glucose pyrophosphohydrolase activity [IDA]ADP-ribose diphosphatase activity [IDA]ADP-ribose pyrophosphohydrolase activity [IDA]NAD binding [IDA]NAD+ diphosphatase activity [IDA]hydrolase activity [ISS]nucleoside-diphosphatase activity [IDA]protein binding [IPI]

Gene Ontology Cellular Component

GG1