BAIT

GRF1

GENERAL REGULATORY FACTOR1-G-BOX FACTOR 14-3-3 HOMOLOG ISOFORM CHI, GF14 CHI, general regulatory factor 1, AT4G09000
14-3-3-like protein GF14 chi
GO Process (1)
GO Function (1)
GO Component (11)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

IDH-III

F8D20.160, F8D20_160, isocitrate dehydrogenase III, AT4G35650
Isocitrate dehydrogenase [NAD] regulatory subunit 3
GO Process (3)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Determining novel functions of Arabidopsis 14-3-3 proteins in central metabolic processes.

Diaz C, Kusano M, Sulpice R, Araki M, Redestig H, Saito K, Stitt M, Shin R

ABSTRACT: BACKGROUND: 14-3-3 proteins are considered master regulators of many signal transduction cascades in eukaryotes. In plants, 14-3-3 proteins have major roles as regulators of nitrogen and carbon metabolism, conclusions based on the studies of a few specific 14-3-3 targets. RESULTS: In this study, extensive novel roles of 14-3-3 proteins in plant metabolism were determined through ... [more]

Unknown Nov. 21, 2011; 5(1);192 [Pubmed: 22104211]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
GRF1 IDH-III
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
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Curated By

  • BioGRID