A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Large-scale protein-protein interaction analysis in Arabidopsis mesophyll protoplasts by split firefly luciferase complementation.

Li JF, Bush J, Xiong Y, Li L, McCormack M

Protein-protein interactions (PPIs) constitute the regulatory network that coordinates diverse cellular functions. There are growing needs in plant research for creating protein interaction maps behind complex cellular processes and at a systems biology level. However, only a few approaches have been successfully used for large-scale surveys of PPIs in plants, each having advantages and disadvantages. Here we present split firefly ... [more]

PLoS ONE Nov. 19, 2011; 6(11);e27364 [Pubmed: 22096563]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

ARF12

Gene Ontology Molecular Function

sequence-specific DNA binding transcription factor activity [ISS]

Gene Ontology Cellular Component

MP

Gene Ontology Biological Process