BAIT

ATS

ABERRANT TESTA SHAPE, KAN4, KANADI 4, MFO20.5, MFO20_5, AT5G42630
probable transcription factor KAN4
GO Process (3)
GO Function (3)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

ETT

ARF3, AUXIN RESPONSE TRANSCRIPTION FACTOR 3, ETTIN, T1B8.30, T1B8_30, AT2G33860
auxin response transcription factor 3
GO Process (5)
GO Function (2)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

ETTIN (ARF3) physically interacts with KANADI proteins to form a functional complex essential for integument development and polarity determination in Arabidopsis.

Kelley D, Arreola A, Gallagher TL, Gasser C

KANADI (KAN) transcription factors promote abaxial cell fate throughout plant development and are required for organ formation during embryo, leaf, carpel and ovule development. ABERRANT TESTA SHAPE (ATS, or KAN4) is necessary during ovule development to maintain the boundary between the two ovule integuments and to promote inner integument growth. Yeast two-hybrid assays identified ETTIN (ETT, or AUXIN RESPONSE FACTOR ... [more]

Unknown Feb. 01, 2012; 0(0); [Pubmed: 22296848]

Throughput

  • Low Throughput

Curated By

  • BioGRID