BAIT

SMARCC2

BAF170, CRACC2, Rsc8
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2
Autism spectrum disorder (ASD) Project
Homo sapiens
PREY

KPNA2

IPOA1, QIP2, RCH1, SRP1alpha
karyopherin alpha 2 (RAG cohort 1, importin alpha 1)
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A lentiviral functional proteomics approach identifies chromatin remodeling complexes important for the induction of pluripotency.

Mak AB, Ni Z, Hewel JA, Chen GI, Zhong G, Karamboulas K, Blakely K, Smiley S, Marcon E, Roudeva D, Li J, Olsen JB, Wan C, Punna T, Isserlin R, Chetyrkin S, Gingras AC, Emili A, Greenblatt J, Moffat J

Protein complexes and protein-protein interactions are essential for almost all cellular processes. Here, we establish a mammalian affinity purification and lentiviral expression (MAPLE) system for characterizing the subunit compositions of protein complexes. The system is flexible (i.e. multiple N- and C-terminal tags and multiple promoters), is compatible with Gateway cloning, and incorporates a reference peptide. Its major advantage is that ... [more]

Mol. Cell Proteomics May. 01, 2010; 9(5);811-23 [Pubmed: 20305087]

Throughput

  • Low Throughput

Ontology Terms

  • cell line: hek-293 cell (BTO:0000007)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SMARCC2 KPNA2
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.9987BioGRID
3071594

Curated By

  • BioGRID