BAIT

AT1G73790

F25P22.21, F25P22_21
mitotic-spindle organizing protein 1A
GO Process (1)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

SPC98

ARABIDOPSIS THALIANA GAMMA TUBULIN COMPLEX PROTEIN 3, ATGCP3, ATSPC98, GAMMA TUBULIN COMPLEX PROTEIN 3, GCP3, MPH15.2, MPH15_2, SPINDLE POLE BODY COMPONENT 98, AT5G06680
spindle pole body component 98
GO Process (1)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

The GCP3-Interacting Proteins GIP1 and GIP2 Are Required for γ-Tubulin Complex Protein Localization, Spindle Integrity, and Chromosomal Stability.

Janski N, Masoud K, Batzenschlager M, Herzog E, Evrard JL, Houlne G, Bourge M, Chaboute ME, Schmit AC

Microtubules (MTs) are crucial for both the establishment of cellular polarity and the progression of all mitotic phases leading to karyokinesis and cytokinesis. MT organization and spindle formation rely on the activity of γ-tubulin and associated proteins throughout the cell cycle. To date, the molecular mechanisms modulating γ-tubulin complex location remain largely unknown. In this work, two Arabidopsis thaliana proteins ... [more]

Unknown Mar. 16, 2012; 0(0); [Pubmed: 22427335]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SPC98 AT1G73790
Reconstituted Complex
Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Low-BioGRID
-
SPC98 AT1G73790
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID