A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Plasma membrane calcium ATPases are important components of receptor-mediated signalling in plant immune responses and development.

Frei Dit Frey N, Mbengue M, Kwaaitaal M, Nitsch L, Altenbach D, Haweker H, Lozano-Duran R, Njo MF, Beeckman T, Huettel B, Borst JW, Panstruga R, Robatzek S

Plasma membrane-resident receptor kinases (RKs) initiate signalling pathways important for plant immunity and development. In Arabidopsis thaliana, the receptor for the elicitor-active peptide epitope of bacterial flagellin, flg22, is encoded by FLS2, which promotes plant immunity. Despite its relevance, the molecular components regulating the FLS2-mediated signalling remain largely unknown. We show that the plasma membrane calcium (Ca2+) ATPase ACA8 forms ... [more]

Unknown Apr. 26, 2012; 0(0); [Pubmed: 22535420]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

FLS2

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP binding [ISS]kinase activity [ISS]protein binding [IPI]protein serine/threonine kinase activity [ISS]transmembrane receptor protein serine/threonine kinase activity [ISS]

Gene Ontology Cellular Component

ACA8