An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

FTIP1 Is an Essential Regulator Required for Florigen Transport.

Liu L, Liu C, Hou X, Xi W, Shen L, Tao Z, Wang Y, Yu H

The capacity to respond to day length, photoperiodism, is crucial for flowering plants to adapt to seasonal change. The photoperiodic control of flowering in plants is mediated by a long-distance mobile floral stimulus called florigen that moves from leaves to the shoot apex. Although the proteins encoded by FLOWERING LOCUS T (FT) in Arabidopsis and its orthologs in other plants ... [more]

PLoS Biol. Apr. 01, 2012; 10(4);e1001313 [Pubmed: 22529749]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
FT AT5G06850	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Liu L (2012)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

FT

Gene Ontology Biological Process

Gene Ontology Molecular Function

phosphatidylethanolamine binding [ISS]protein binding [IPI]

Gene Ontology Cellular Component

AT5G06850