AS1
Gene Ontology Biological Process
- asymmetric cell division [IMP]
- defense response to bacterium [IMP]
- defense response to fungus [IMP]
- leaf formation [IGI]
- leaf morphogenesis [IMP]
- negative regulation of transcription, DNA-templated [IDA]
- polarity specification of adaxial/abaxial axis [IMP]
- regulation of innate immune response [IMP]
- response to auxin [IEP]
- response to cadmium ion [IEP]
- response to gibberellin [IEP]
- response to jasmonic acid [IEP]
- response to salicylic acid [IEP]
- response to salt stress [IEP]
- response to virus [IMP]
Gene Ontology Molecular Function
CAM1
Gene Ontology Biological Process
Gene Ontology Molecular Function
PCA
A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.
Publication
The transcriptional repressor activity of ASYMMETRIC LEAVES1 is inhibited by direct interaction with calmodulin in Arabidopsis.
Calmodulin (CaM), a key Ca(2+) sensor, regulates diverse cellular processes by modulating the activity of a variety of enzymes and proteins. However, little is known about the biological function of CaM in plant development. In this study, an ASYMMETRIC LEAVES1 (AS1) transcription factor was isolated as a CaM binding protein. AS1 contains two putative CaM-binding domains (CaMBDs) at the N-terminus. ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| AS1 CAM1 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID