SMAP1
Gene Ontology Biological Process
Gene Ontology Molecular Function
COP8
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
SMALL ACIDIC PROTEIN 1 acts with RUB modification components, the COP9 signalosome and AXR1, to regulate growth and development of Arabidopsis thaliana.
Previously, a dysfunction of the SMALL ACIDIC PROTEIN 1 (SMAP1) gene was identified as the cause of the antiauxin resistant 1 (aar1) mutant of Arabidopsis. SMAP1 is involved in the response pathway of synthetic auxin, 2,4-dichlorophenoxyacetic acid (2,4-D), and functions upstream of the AUXIN (AUX)/INDOLE-3-ACETIC ACID (IAA)-proteins degradation step in auxin signaling. However, the exact mechanism by which SMAP1 functions ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SMAP1 COP8 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - |
Curated By
- BioGRID