BAIT

PLDDELTA

ARABIDOPSIS THALIANA PHOSPHOLIPASE D DELTA, ATPLDDELTA, F4B14.60, F4B14_60, PHOSPHOLIPASE D, phospholipase D delta, AT4G35790
phospholipase D delta
Arabidopsis thaliana (Columbia)
PREY

GAPC1

GAPC, GAPC-1, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C SUBUNIT, T6K12.26, T6K12_26, glyceraldehyde-3-phosphate dehydrogenase C subunit 1, AT3G04120
glyceraldehyde-3-phosphate dehydrogenase C subunit 1
Arabidopsis thaliana (Columbia)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Cytosolic Glyceraldehyde-3-Phosphate Dehydrogenases Interact with Phospholipase Dδ to Transduce Hydrogen Peroxide Signals in the Arabidopsis Response to Stress.

Guo L, Devaiah SP, Narasimhan R, Pan X, Zhang Y, Zhang W, Wang X

Reactive oxygen species (ROS) are produced in plants under various stress conditions and serve as important mediators in plant responses to stresses. Here, we show that the cytosolic glycolytic enzymes glyceraldehyde-3-phosphate dehydrogenases (GAPCs) interact with the plasma membrane-associated phospholipase D (PLDδ) to transduce the ROS hydrogen peroxide (H(2)O(2)) signal in Arabidopsis thaliana. Genetic ablation of PLDδ impeded stomatal response to ... [more]

Unknown May. 15, 2012; 0(0); [Pubmed: 22589465]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PLDDELTA GAPC1
Reconstituted Complex
Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Low-BioGRID
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Curated By

  • BioGRID