BAIT
SF3A2
PRP11, PRPF11, SAP62, SF3a66
splicing factor 3a, subunit 2, 66kDa
GO Process (5)
GO Function (2)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
SRRM1
160-KD, POP101, SRM160
serine/arginine repetitive matrix 1
GO Process (8)
GO Function (1)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Composition and three-dimensional EM structure of double affinity-purified, human prespliceosomal A complexes.
Little is known about the higher-order structure of prespliceosomal A complexes, in which pairing of the pre-mRNA's splice sites occurs. Here, human A complexes were isolated under physiological conditions by double-affinity selection. Purified complexes contained stoichiometric amounts of U1, U2 and pre-mRNA, and crosslinking studies indicated that these form concomitant base pairing interactions with one another. A complexes contained nearly ... [more]
EMBO J. Mar. 21, 2007; 26(6);1737-48 [Pubmed: 17332742]
Throughput
- Low Throughput
Curated By
- BioGRID