A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Arabidopsis Fused kinase and the Kinesin-12 subfamily constitute a signalling module required for phragmoplast expansion.

Oh SA, Allen T, Kim GJ, Sidorova A, Borg M, Park SK, Twell D

The conserved Fused kinase plays vital but divergent roles in many organisms from Hedgehog signalling in drosophila to polarisation and chemotaxis in Dictyostelium. Previously we have shown that Arabidopsis Fused kinase termed TWO-IN-ONE (TIO) is essential for cytokinesis in both sporophytic and gametophytic cell types. Here using in vivo imaging of GFP-tagged microtubules in dividing microspores we show that TIO ... [more]

Unknown Jun. 18, 2012; 0(0); [Pubmed: 22709276]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
FU KINESIN-12B	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Oh SA (2012)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

FU

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]protein serine/threonine kinase activity [ISS]

Gene Ontology Cellular Component

KINESIN-12B