RCD1
Gene Ontology Biological Process
- defense response to bacterium, incompatible interaction [IMP]
- embryo development ending in seed dormancy [IMP]
- ethylene-activated signaling pathway [IMP]
- jasmonic acid mediated signaling pathway [IMP]
- lateral root morphogenesis [IMP]
- nitric oxide biosynthetic process [IMP]
- programmed cell death [IMP]
- regulation of reactive oxygen species metabolic process [IMP]
- response to ethylene [IMP]
- response to hydrogen peroxide [IMP]
- response to osmotic stress [IMP]
- response to oxidative stress [IGI]
- response to ozone [IMP]
- response to salt stress [IMP]
- response to superoxide [IMP]
- response to water deprivation [IMP]
Gene Ontology Molecular Function
GCL1
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PCA
A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.
Publication
Uncovering Arabidopsis membrane protein interactome enriched in transporters using mating-based split ubiquitin assays and classification models.
High-throughput data are a double-edged sword; for the benefit of large amount of data, there is an associated cost of noise. To increase reliability and scalability of high-throughput protein interaction data generation, we tested the efficacy of classification to enrich potential protein-protein interactions. We applied this method to identify interactions among Arabidopsis membrane proteins enriched in transporters. We validated our ... [more]
Throughput
- High Throughput
Curated By
- BioGRID