PFT1
Gene Ontology Biological Process
- defense response to fungus [IMP]
- jasmonic acid mediated signaling pathway [IMP]
- positive regulation of defense response [IMP]
- positive regulation of flower development [IMP]
- positive regulation of transcription, DNA-templated [IMP]
- red, far-red light phototransduction [IMP]
- regulation of flower development [IMP]
- response to far red light [IMP]
- response to red light [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- mediator complex [IDA]
- nucleus [IDA, ISM]
WRKY10
Gene Ontology Biological Process
Gene Ontology Molecular Function
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
MED25 Acts as an Integrative Hub for the Regulation of Jasmonate-Responsive Gene Expression in Arabidopsis.
The PHYTOCHROME AND FLOWERING TIME1 (PFT1) gene encoding the MEDIATOR25 (MED25) subunit of the eukaryotic Mediator complex is a positive regulator of jasmonate- (JA) responsive gene expression in Arabidopsis thaliana. Based on the function of the Mediator complex as a bridge between DNA-bound transcriptional activators and the RNA polymerase II complex, MED25 has been hypothesized to function in association with ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| WRKY10 PFT1 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID