Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Genome-wide YFP fluorescence complementation screen identifies new regulators for telomere signaling in human cells.

Lee OH, Kim H, He Q, Baek HJ, Yang D, Chen LY, Liang J, Chae HK, Safari A, Liu D, Songyang Z

Detection of low-affinity or transient interactions can be a bottleneck in our understanding of signaling networks. To address this problem, we developed an arrayed screening strategy based on protein complementation to systematically investigate protein-protein interactions in live human cells, and performed a large-scale screen for regulators of telomeres. Maintenance of vertebrate telomeres requires the concerted action of members of the ... [more]

Mol. Cell Proteomics Feb. 01, 2011; 10(2);M110.001628 [Pubmed: 21044950]

Throughput

High Throughput

Additional Notes

BifC

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
TERF2IP SMARCC2	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Go CD (2021)	High	17.33	BioGRID	3009221

Curated By

BioGRID

Interaction Summary

TERF2IP

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]telomeric DNA binding [TAS]

Gene Ontology Cellular Component

SMARCC2

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]nucleosomal DNA binding [IDA]protein binding [IPI]transcription coactivator activity [NAS]