An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Photoactivated phytochromes interact with HEMERA and promote its accumulation to establish photomorphogenesis in Arabidopsis.

Galvao RM, Li M, Kothadia SM, Haskel JD, Decker PV, Van Buskirk EK, Chen M

Plant development is profoundly regulated by ambient light cues through the red/far-red photoreceptors, the phytochromes. Early phytochrome signaling events include the translocation of phytochromes from the cytoplasm to subnuclear domains called photobodies and the degradation of antagonistically acting phytochrome-interacting factors (PIFs). We recently identified a key phytochrome signaling component, HEMERA (HMR), that is essential for both phytochrome B (phyB) localization ... [more]

Genes Dev. Aug. 15, 2012; 26(16);1851-63 [Pubmed: 22895253]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
PTAC12 PHYA	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Galvao RM (2012)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

PTAC12

Gene Ontology Biological Process

Gene Ontology Cellular Component

PHYA

Gene Ontology Biological Process

Gene Ontology Molecular Function

G-protein coupled photoreceptor activity [ISS]far-red light photoreceptor activity [IMP]identical protein binding [IPI]protein binding [IPI]protein histidine kinase activity [ISS]red or far-red light photoreceptor activity [TAS]signal transducer activity [ISS]