BAIT

PAT1

MIF21.4, MIF21_4, phytochrome a signal transduction 1, AT5G48150
GRAS family transcription factor
GO Process (1)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

SCL21

F1O13.2, F1O13_2, SCARECROW-like 21, AT2G04890
scarecrow-like protein 21
GO Process (1)
GO Function (2)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Two GRAS Proteins, SCL21 and PAT1, function cooperatively in the Phytochrome A Signal Transduction.

Torres-Galea P, Hirtreiter B, Bolle C

Photoreceptors, especially the far-red light absorbing phytochrome A, play a crucial role in early seedling development triggering the transition from etiolated to photomorphogenic growth. Here, we describe the biological function of two GRAS proteins from Arabidopsis thaliana, SCL21 and PAT1, which are specifically involved in phytochrome A signal transduction. Loss-of-function mutants show an elongated hypocotyl under far-red light and are ... [more]

Plant Physiol. Oct. 30, 2012; 0(0); [Pubmed: 23109688]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PAT1 SCL21
Reconstituted Complex
Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Low-BioGRID
-

Curated By

  • BioGRID