An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Proteasome-Mediated Turnover of Arabidopsis MED25 Is Coupled to the Activation of FLOWERING LOCUS T Transcription.

Inigo S, Giraldez A, Chory J, Cerdan P

The Mediator complex is a >1 MDa complex, composed of about 30 subunits found in most eukaryotes, whose main role is to transmit signals from DNA bound transcription factors to RNA Polymerase II. The proteasome is emerging as an important regulator of transcription during both initiation and elongation. It is increasing the number of cases where the proteolysis of transcriptional ... [more]

Plant Physiol. Sep. 19, 2012; 0(0); [Pubmed: 22992513]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
AT4G34040 PFT1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Inigo S (2012)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

AT4G34040

Gene Ontology Molecular Function

zinc ion binding [ISS]

Gene Ontology Cellular Component

PFT1

Gene Ontology Biological Process