HK5
Gene Ontology Biological Process
- cellular response to hydrogen peroxide [IMP]
- cellular response to molecule of bacterial origin [IMP]
- cellular response to nitric oxide [IMP]
- cytokinin-activated signaling pathway [TAS]
- negative regulation of abscisic acid-activated signaling pathway [IMP]
- negative regulation of ethylene-activated signaling pathway [IMP]
- regulation of stomatal closure [IMP]
- root development [IMP]
Gene Ontology Molecular Function
AHP5
Gene Ontology Biological Process
Gene Ontology Molecular Function
PCA
A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.
Publication
Structure-function analysis of Arabidopsis thaliana histidine kinase AHK5 bound to its cognate phosphotransfer protein AHP1.
The multi-step phosphorelay (MSP) system defines a key signal transduction pathway in plants and many eukaryotes. In this system, external stimuli first lead to the activation of a histidine kinase, followed by transfer of a phosphoryl group from the receiver domain of the kinase (HK(RD)) to downstream, cytosolic phosphotransfer proteins (HPs). In order to establish the determinants of specificity for ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID