BAIT

PEX7

ARABIDOPSIS PEROXIN 7, ATPEX7, F28N24.6, F28N24_6, PEROXISOMAL TARGETING SIGNAL TYPE 2 RECEPTOR, peroxin 7, AT1G29260
peroxin 7
GO Process (1)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

RAB8A

ARA-3, ARA3, ATRAB8A, ATRABE1C, RAB GTPase homolog 8A, SMALL GTP-BINDING PROTEIN, AT3G46060
small GTP-binding protein ARA3
GO Process (1)
GO Function (2)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Proteomic Analysis Reveals that the Rab GTPase RabE1c is Involved in the Degradation of the Peroxisomal Protein Receptor PEROXIN 7.

Cui S, Fukao Y, Mano S, Yamada K, Hayashi M, Nishimura M

The biogenesis of peroxisomes is mediated by peroxins (PEXs). PEX7 is a cytosolic receptor that imports peroxisomal targeting signal type 2 (PTS2)-containing proteins. Although PEX7 is important for protein transport, the mechanisms that mediate its function are unknown. In this study, we performed proteomic analysis to identify PEX7-binding proteins using transgenic Arabidopsis expressing green fluorescent protein (GFP)-tagged PEX7. Our analysis ... [more]

J. Biol. Chem. Jan. 07, 2013; 0(0); [Pubmed: 23297417]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PEX7 RAB8A
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-

Curated By

  • BioGRID