An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Structure of the Atg12-Atg5 conjugate reveals a platform for stimulating Atg8-PE conjugation.

Noda NN, Fujioka Y, Hanada T, Ohsumi Y, Inagaki F

Atg12 is conjugated to Atg5 through enzymatic reactions similar to ubiquitination. The Atg12-Atg5 conjugate functions as an E3-like enzyme to promote lipidation of Atg8, whereas lipidated Atg8 has essential roles in both autophagosome formation and selective cargo recognition during autophagy. However, the molecular role of Atg12 modification in these processes has remained elusive. Here, we report the crystal structure of ... [more]

EMBO Rep. Dec. 14, 2012; 0(0); [Pubmed: 23238393]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

ATG3

Gene Ontology Biological Process

Gene Ontology Cellular Component