A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

RAD5a ubiquitin ligase is involved in ubiquitination of Arabidopsis thaliana proliferating cell nuclear antigen.

Strzalka W, Bartnicki F, Pels K, Jakubowska A, Tsurimoto T, Tanaka K

The proliferating cell nuclear antigen (PCNA) is post-translationally modified by ubiquitin in yeast and mammalian cells. It is widely accepted that in yeast mono- and polyubiquitinated PCNA is involved in distinct pathways of DNA postreplication repair. This study showed an interaction between plant ubiquitin and PCNA in the plant cell. Using different approaches, it was demonstrated that Arabidopsis RAD5a ubiquitin ... [more]

J. Exp. Bot. Jan. 10, 2013; 0(0); [Pubmed: 23314815]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

PCNA2

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [ISS]DNA polymerase processivity factor activity [ISS]protein binding [IPI]

Gene Ontology Cellular Component

UBQ3