BAIT

RIC1

F4P9.23, F4P9_23, ROP-interactive CRIB motif-containing protein 1, AT2G33460
ROP-interactive CRIB motif-containing protein 1
GO Process (4)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

ERH3

AAA1, ARABIDOPSIS THALIANA KATANIN SMALL SUBUNIT, ATKSS, ATKTN1, BOT1, BOTERO 1, CAD ATPASE, ECTOPIC ROOT HAIR 3, F5I6.10, F5I6_10, FAT ROOT, FRA2, FRAGILE FIBER 2, FRC2, FTR, FURCA2, KATANIN 1, KTN1, LUE1, AT1G80350
ATPase katanin p60
GO Process (5)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Rho GTPase Signaling Activates Microtubule Severing to Promote Microtubule Ordering in Arabidopsis.

Lin D, Cao L, Zhou Z, Zhu L, Ehrhardt D, Yang Z, Fu Y

BACKGROUND: Ordered cortical microtubule (MT) arrays play a critical role in the spatial control of cell division and expansion and are essential for plant growth, morphogenesis, and development. Various developmental, hormonal, and mechanical signals and a large number of MT-associated proteins are known to impact cortical MT organization, but the underlying mechanisms remain poorly understood. Our previous studies show that ... [more]

Curr. Biol. Feb. 05, 2013; 0(0); [Pubmed: 23394835]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RIC1 ERH3
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
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Curated By

  • BioGRID