An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

HONSU, a protein phosphatase 2C, Regulates Seed Dormancy by Inhibiting Abscisic Acid Signaling in Arabidopsis.

Kim W, Lee Y, Park J, Lee N, Choi G

Seed dormancy, a seed status that prohibits germination even in the presence of inductive germination signals, is a poorly understood process. To identify molecular components that regulate seed dormancy, we screened T-DNA insertion lines and identified a mutant designated honsu (hon). HON loss-of-function mutants display deep seed dormancy, whereas HON-overexpressing lines display shallow seed dormancy. HON encodes a seed-specific group ... [more]

Plant Cell Physiol. Feb. 01, 2013; 0(0); [Pubmed: 23378449]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
HAI2 PYR1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Altmann M (2020)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

PYR1

Gene Ontology Biological Process

Gene Ontology Molecular Function

abscisic acid binding [IDA]protein binding [IPI]protein homodimerization activity [IDA, IPI]receptor activity [IDA, IMP]

Gene Ontology Cellular Component

HAI2