A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

The ULT1 and ULT2 trxG Genes Play Overlapping Roles in Arabidopsis Development and Gene Regulation.

Monfared MM, Carles CC, Rossignol P, Pires HR, Fletcher JC

The epigenetic regulation of gene expression is critical for ensuring the proper deployment and stability of defined genome transcription programs at specific developmental stages. The cellular memory of stable gene expression states during animal and plant development is mediated by the opposing activities of Polycomb group (PcG) factors and trithorax group (trxG) factors. Yet despite their importance, only a few ... [more]

Mol Plant Feb. 27, 2013; 0(0); [Pubmed: 23446032]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
ULT1 ULT2	FRET FRET An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.	Pires HR (2014)	Low	-	BioGRID	-
ULT2 ULT1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Pires HR (2014)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

ULT1

Gene Ontology Biological Process

Gene Ontology Cellular Component

ULT2