DCL1
Gene Ontology Biological Process
- RNA processing [ISS]
- cytokinesis by cell plate formation [IMP]
- embryonic pattern specification [IMP]
- flower development [TAS]
- mRNA cleavage involved in gene silencing by miRNA [IMP]
- primary miRNA processing [TAS]
- production of lsiRNA involved in RNA interference [IMP]
- production of miRNAs involved in gene silencing by miRNA [IGI]
- production of siRNA involved in RNA interference [IMP]
- production of ta-siRNAs involved in RNA interference [IMP]
- regulation of seed maturation [IMP]
- seed morphogenesis [IMP]
- suspensor development [IMP]
- vegetative to reproductive phase transition of meristem [IMP]
- virus induced gene silencing [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
AT1G07705
Gene Ontology Cellular Component
- chloroplast [ISM]
- nucleus [ISM]
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
NOT2 Proteins Promote Polymerase II-Dependent Transcription and Interact with Multiple MicroRNA Biogenesis Factors in Arabidopsis.
MicroRNAs (miRNAs) play key regulatory roles in numerous developmental and physiological processes in animals and plants. The elaborate mechanism of miRNA biogenesis involves transcription and multiple processing steps. Here, we report the identification of a pair of evolutionarily conserved NOT2_3_5 domain-containing-proteins, NOT2a and NOT2b (previously known as At-Negative on TATA less2 [NOT2] and VIRE2-INTERACTING PROTEIN2, respectively), as components involved in ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| AT1G07705 DCL1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID